Past and current scientific interest focused on:

a) Wastewater reuse, reclaimed water technologies and applications, engineered wetlands, water infrastructure, and wastewater treatment,

b) Human opportunistic pathogens with particular emphasis on Cryptosporidium, Giardia, and human-virulent microsporidia,

c) Waterborne pathogens (drinking, waste, and recreational waters),

d) Molecular epidemiology of enteric pathogens,

e) Immunodiagnosis of parasitic infections,

f) Human zoonotic trematodiases.

Specific interests regarding Cryptosporidium, Giardia, and human-virulent microsporidia include:

Recovery of oocysts, cysts, and spores from environmental samples; Determination of pathogen infectivity; Modes of environmental contamination and pathogen transmission; Prophylaxis and therapy.

Environmental Health Sciences, enteric diseases, waterborne parasites, Cryptosporidium, parasite transmission, Giardia, medical parasitology, human-virulent microsporidia, tropical diseases

Certificate of Recognition, United Way of Maryland

Certificatec of Excellence, Morris Animal Foundation

Am Soc Parasitol Travel Award Award, 1994

Am Water Work Assoc Res Foundation Award, 2003

U.S. Department of Commerce Unit Recognition Award, 2004

Fulbright Foreign Specialist Award, 2005

References (selected from 250 peer-reviewed publications)

Graczyk, T. K., and F. E. Lucy. 2007. Quality of reclaimed waters; a public health need for source-tracking of wastewater-derived protozoan enteropathogens in engineered wetlands. Transactions of the Royal Society of Tropical Medicine and Hygiene 101: 532-533.

Graczyk, T. K., F. E. Lucy, L. Tamang, and A. Miraflor. 2007. Human enteropathogen load in activated sewage sludge and corresponding sewage sludge-end products. Applied and Environmental Microbiology 73 (6): 2013-2015.

Graczyk, T. K., M. Johansson, L. Tamang, G. S. Visvesvara, L. S. Moura, A. J. DaSilva, A. S. Girouard, and O. Matos. 2007. Retrospective species identification of microsporidian spores in diarrhetic fecal samples from HIV/AIDS patients by multiplexed fluorescent in situ hybridization (FISH). Journal of Clinical Microbiology 45 (4); 1255-1260.

Graczyk, T. K., E. J. Lewis, G. Glass, A. J. DaSilva, L. Tamang, A. S. Girouard, F. C. Curriero. 2007. Quantitative assessment of viable Cryptosporidium parvum load in commercial oysters (Crassostrea virginica) in the Chesapeake Bay. Parasitology Research 100 (2): 247-253.

Graczyk, T. K., A. S. Girouard, L. Tamang, S. P. Nappier, and K. J. Schwab. 2006. Recovery, bioaccumulation, and inactivation of human waterborne pathogens by the Chesapeake Bay non-native oyster, Crassostrea ariakensis. Applied and Environmental Microbiology 72, (5); 3390-3395.

Graczyk, T. K., C. J. Shiff, L. Tamang, F. Munsaka, A. M. Beitin, and W. J. Moss. 2005. The association of Blastocystis hominis and Endolimax nana with diarrhea in Zambian school-age children. Parasitology Research 98: 38-43.

Graczyk, T. K. 2005. Is Giardia a living fossil? Trends in Parasitology 21: 104-107.

Graczyk, T. K., R. Knight, and L. Tamang 2005. Mechanical transmission of human protozoan parasites by insects. Clinical Microbiology Reviews 18: 128-132.

Graczyk, T. K., D. B. Conn, F. Lucy, D. Minchin, L. Tamang, L. N. S. Moura, and A. J. DaSilva. 2004. Human waterborne parasites in zebra mussels (Dreissena polymorpha) from the Shannon River drainage, Ireland. Parasitology Research 93 (5): 389-391.

Graczyk, T. K., and M. R. Cranfield. 2003. Coprophagy and intestinal parasites: implications to Mountain gorillas (Gorilla gorilla beringei) of the Virunga Mountains and Bwindi Impenetrable Forest. Primate Conservation 19: 35-41.

Graczyk, T. K. 2003. Human waterborne parasites in molluscan shellfish. Journal of Parasitology 89; 557-561.

Graczyk, T. K., B. H. Grimes, R. Knight, A. J. DaSilva, N. J. Pieniazek, and D. A. Veal. 2003. Combined FISH and mAb detection of Cryptosporidium parvum and Giardia lamblia carried by synanthropic flies. American Journal of Tropical Medicine and Hygiene 68, (2): 228-232.

Graczyk, T. K., J. Bosco-Nizeyi, A. J. DaSilva, I. N. S. Moura N. J. Pieniazek. M. R. Cranfield, and H. D. A. Lindguist. 2002. A single genotype of Encephalitozoon intestinalis infects free-ranging gorillas and people sharing their habitats, Uganda. Parasitology Research 88, (10): 926-931.

Graczyk, T. K., J. B. Nizeyi, B. Ssebide, R. C. A. Thompson, C. Read, and M. R. Cranfield. 2002. Anthropozoonotic Giardia duodenalis Genotype (Assemblage) A infections in habitas of free-ranging human-habituated gorillas, Uganda. Journal of Parasitology 88, (5): 905-909.

Graczyk, T. K., R. H. Gilman, and B. Fried. 2001. Fasciolopsiasis: is it a controllable food-borne disease? Parasitology Research 87, (1): 80-83.

Graczyk, T. K., R. Knight, R. H. Gilman, and M. R. Cranfield. 2001. The role of non-biting flies in the epidemiology of human infectious diseases. Microbes and Infection 3 (3): 231-235.

Graczyk, T. K., D. J. Marcogliese, Y. deLafontaine, A. J. DaSilva, B. Mhangami-Ruwende, and N. J. Pieniazek. 2001. Cryptosporidium parvum oocysts in zebra mussels (Dreissena polymorpha): evidence from the St. Lawrence River. Parasitology Research 87 (3): 231-234.

Graczyk, T. K., A. J. DaSilva, M. R. Cranfield, J. Bosco Nizeyi, G. R. N. N. Kalema, and N. J. Pieniazek. 2001. Cryptosporidium parvum Genotype 2 infections in free-ranging mountain gorillas (Gorilla gorilla beringei) of the Bwindi Impenetrable National Park, Uganda. Parasitology Research 87 (5): 368-370.

Graczyk, T. K., M. R. Cranfield, and R. Fayer. 1997. Recovery of waterborne oocysts of Cryptosporidium parvum from water samples by the membrane-filter dissolution method. Parasitology Research 83: 121-125.

Graczyk, T. K., R. Fayer, M. R. Cranfield, and R. Owens 1997. Cryptosporidium parvum oocysts recovered from water by the membrane filter dissolution method retain their infectivity. Journal of Parasitology 83 (1) 111-114.

Graczyk, T. K., R. Fayer, and M. R. Cranfield. 1997. Zoonotic potential of cross-transmission of Cryptosporidium parvum: implications for waterborne cryptosporidiosis. Parasitology Today 13: 348-351.

Graczyk, T. K., M. K. Cranfield, R. Fayer, J. Trout, and H. J. Goodale. 1997. Infectivity of Cryptosporidium parvum oocysts is retained upon intestinal passage through a migratory waterfowl species (Canada goose, Branta canadensis). Tropical Medicine & International Health 2: 341-347.

Graczyk, T. K., Fayer. R., Cranfield, M. R., and D. B. Conn. 1977. In vitro interactions of the Asian freshwater clam (Corbicula fluminea) hemocytes and Cryptosporidium parvum oocysts. Applied and Environmental Microbiology, 63: 2910-2912.

Graczyk, T. K., M. R. Cranfield, and R. Fayer. 1996. Evaluation of commercial enzyme immunoassay (EIA) and immunofluorescent antibody (IFA) tests kits for detection of Cryptosporidium oocysts other than Cryptosporidium parvum. The American Journal of Tropical Medicine and Hygiene 53: 274-279.

Graczyk, T. K., M. R. Cranfield, R. Fayer, and M. S. Anderson. 1996. Viability and infectivity of Cryptosporidium parvum oocysts are retained upon intestinal passage through a refractory avian host. Applied and Environmental Microbiology 62: 3234-3237.

Books:

Fried, B., and T. K. Graczyk. 1997. Advances in Trematode Biology, CRC Press, Inc., Boca Raton, Florida; 446 p.

Fried B., and T. K. Graczyk. 2000. Echinostomes as Experimental Models for Biological Research. Kluwer Academic Publishers, Dordrecht, The Netherlands, 273 p.

Galaktionov, K., B. Fried, and T. K. Graczyk. 2003. The Origin and Evolution of Thrematode Life Cycles. Kluwer Academic Publishers, Dordrecht, The Netherlands; 592 p.