The research conducted in my laboratory focuses on molecular biomonitoring of genotoxic agents and genetic polymorphisms associated with their metabolism. The general theme is the assessment of carcinogen metabolites and genetic damage in human populations with known or suspected exposure to various occupational or environmental carcinogens. This approach has widespread application in assessing previous exposure to carcinogens and is increasingly being used as a predictor of ultimate disease risk. The ability to measure carcinogen metabolites and genetic damage in human tissues allows one to address a variety of public health issues related to exposure assessment and individual susceptibility to cancer. Furthermore, elucidating the spectrum of common polymorphisms in carcinogen metabolizing and other genes associated with disease outcome provides an approach to assessing individual susceptibility to cancer.

Environmental health sciences, molecular biomonitoring, genotoxic agents, carcinogens, genetic polymorphisms, carcinogen metabolites, genetic damage in human populations, molecular epidemiology

International Agency for Research on Cancer Research Fellow (UK)

Dale Research Fellow (Paterson Laboratories, UK)

Howard Fox Lecture, NYU Medical Center, New York

Keynote Lecture, International Symposium on Biological Monitoring, Seoul, Korea

Member, European Academy of Sciences, Biomedical Sciences Section

Laken SJ, Jackson PE, Kinzler KW, Vogelstein B, Strickland PT, Groopman JD, Friesen MD. Genotyping by mass spectrometric analysis of short DNA fragments. Nature Biotechnol 16:1352-1356, 1998.

Strickland PT, Kang DH. Urinary 1-hydroxypyrene and other PAH metabolites as biomarkers of exposure to environmental PAH in air particulate matter. Toxicol Let 108:191-199, 1999.

Sithisarankul P, Weaver VM, Davoli CT, Strickland PT. Urinary 5-aminolevulinic acid in lead-exposed children. Biomarkers 4:281-289, 1999.

Lee CK, Cho SH, Kang JW, Lee SJ, Ju YS, Sung JH, Strickland PT, Kang DH. Comparison of three analytical methods for 1-hydroxypyrene-glucuronide in urine after non-occupational exposure to polycyclic aromatic hydrocarbons. Toxicol Let 108:209-215, 1999.

Northridge ME, Yankura J, Kinney P, Santella RM, Shepard P, Riojas Y, Aggarwal M, Strickland P, The Earth Crew. Diesel exhaust exposure among adolescents in Harlem: A community-driven study. Am J Public Health 89:998-1002, 1999.

Sithisarankul P, Cadorette M, Davoli CT, Serwint JR, Chisolm JJ, Strickland PT. Plasma 5-amino- levulinic acid concentration and lead exposure in children. Environ Res 80:41-49, 1999.

Helzlsouer KJ, Alberg AJ, Huang HY, Hoffman S, Strickland PT, Brock JW, Burse VW, Needham LL, Bell DA, Lavigne J, Yager JD, Comstock GW. Serum concentrations of organochlorine compounds and the subsequent development of breast cancer. Cancer Epidemiol Biomarkers Preven 8:525-532, 1999.

Zavala AG, Lancaster T, Groopman JD, Strickland PT, Chandrasegaran S. Phage display of ScFv peptides recognizing thymidine(6-4)thymidine photoproduct. Nucleic Acids Res 28:e24, 2000.

Carlson MA, Bargeron CB, Benson RC, Fraser AB, Phillips TE, Velky JT, Groopman JD, Strickland PT. An automated handheld biosensor for aflatoxin. Biosensors Bioelectron 14:841-848, 2000.

Park SK, Yoo KY, Lee SJ, Kim SU, Ahn SH, Noh DY, Choe KJ, Strickland PT, Hirvonen A, Kang DH. Alcohol consumption, glutathione S-transferase M1 and T1 genetic polymorphisms and breast cancer risk. Pharmacogenetics 10:301-309, 2000.

Cho SH, Kang JW, Ju YS, Sung JH, Lee CK, Lee YS, Strickland PT, Kang DH. Use of urinary PAH metabolites to assess PAH exposure intervention among coke oven workers. J Occup Health 42:138-143, 2000.

Phillips TE, Bargeron CB, Benson RC, Carlson MA, Fraser AB, Groopman JD, Ko HW, Strickland PT, Velky JT. Development of an automated, handheld, immunoaffinity fluorometric biosensor. Proc SPIE 3913:186-192, 2000.

Scholl PF, Bargeron CB, Phillips TE, Wong T, Sala Abubaker, Groopman JD, Strickland PT, Benson RC. Immunoaffinity based phosphorescent sensor platform for the detection of bacterial spores. Proc SPIE 3913:204-214, 2000.

Roth MJ, Qiao YL, Rothman N, Tangrea JA, Dawsey SM, Wang GQ, Cho SH, Kang DH, Taylor PR, Strickland PT. High urine 1-hydroxypyrene glucuronide concentrations in Linxian, China, an area of high risk for squamous esophageal cancer. Biomarkers 6:381-386, 2001.

Strickland PT, Qian Z, Friesen M, Rothman N, Sinha R. Measurement of 2-amino-1-methyl-6-phenylimidazo (4,5-b)pyridine (PhIP) in acid-hydrolyzed urine by high performance liquid chromatography with fluorescence detection. Biomarkers 6:313-325, 2001

Friesen M, Rothman N, Strickland PT. Concentration of 2-amino-1-methyl-6-phenylimidazo-(4,5-b)- pyridine (PhIP) in urine and alkali-hydrolyzed urine after consumption of charbroiled beef. Cancer Letters 173:43-51, 2001.

Wang JS, Abubaker S, He X, Strickland PT, Groopman JD. Development of an aflatoxin B1-lysine adduct specific monoclonal antibody for human exposure studies. Appld Environ Microbiol 67:2712-2717,2001

Yim DS, Park SK, Yoo KY, Yoon KS, Chung HH, Ahn SH, Noh DY, Choe KJ, Jang IJ, Shin SG, Strickland PT, Hirvonen A, Kang DH. Relationship between the val158met polymorphism of catechol O-methyl transferase and breast cancer risk. Pharmacogenetics 11:279-286, 2001.

Goodman JE, Lavigne JA, Wu K, Helzlsouer KJ, Strickland PT, Selhub J, Yager JD. COMT genotype, micronutrients in the folate metabolic pathway and risk for breast cancer. Carcinogenesis 22:1661-1665, 2001.

Whitmore SE, Potten CS, Chadwick CA, Strickland PT, Morison WL. Effect of photoreactivating light on UV radiation-induced alterations in human skin. Photodermatol Photoimmunol Photomed 17:213-217, 2001.

Strickland PT, Qian Z, Friesen M, Rothman N, Sinha R. Metabolites of 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) in human urine after consumption of charbroiled or fried meat. Mutation Research 506:163-173, 2002.

Lee KH, Lee JM, Ha M, Choi JW, Cho SH, Hwang ES, Park CG, Strickland PT, Hirvonen A, Kang DH. Influence of polymorphism of GSTM1 gene on association between glycophorin A mutant frequency and urinary PAH metabolites in incineration workers. J Toxicol Environ Health 65:355-363, 2002.

Lee JM, Kang DH, Lee KH, Masayoshi I, Zhang JS, Tomokuni K, Huang ES, Park CG, Ha M, Kim SG, Han SB, Choi JW, Lee E, Jang JY, Strickland PT, Hirvonen A, Cho SH. Influence of GSTM1 genotype on association between aromatic DNA adducts and urinary PAH metabolites in incineration workers. Mutation Research 514:213-221, 2002.

Kim SU, Park SK, Yoo KY, Yoon KS, Choi JY, Seo JS, Park WY, Kim JH, Noh DY, Ahn SH, Choe KJ, Strickland PT, Hirvonen A, Kang DH. XRCC1 genetic polymorphism and breast cancer risk. Pharmacogenetics 12:335-338, 2002.

Ishibe N, Sinha R, Hein DW, Kulldorff M, Strickland P, Fretland AJ, Chow WH, Kadlubar FF, Lang NP, Rothman N. Genetic polymorphisms in heterocyclic amine metabolism and risk of colorectal adenomas. Pharmacogenetic 12:145-150, 2002.

Sutter CH, Qian Z, Mammen JS, Strickland PT, Sutter TR. Genotyping human cytochrome P450 1B1 variants. Methods Enzymol 375:53-58, 2002.

Cantor KP, Strickland PT, Brock JW, Bush D, Helzlsouer K, Needham LL, Zahm SH, Comstock GW, Rothman N. Risk of non-Hodgkin's lymphoma and pre-diagnostic serum organchlorines: ß-hexachlorocyclohexane, chlordane/heptachlor-related compounds, dieldrin, and hexachloro-benzene. Env Health Perspec 111:179-183, 2003

Park SK, Kang DH, Noh DY, Kang HJ, Ahn SH, Choe KJ, Strickland PT, Hirvonen A, Yoo KY. Reproductive factors, glutathione S-transferase M1 and T1 genetic polymorphisms and breast cancer risk. Breast Cancer Research Treatment 78:89-96, 2003.

Lee KH, Ichiba M, Zhang JS, Tomokuni K, Hong YC, Ha M, Lee JM, Ha M, Kwon HJ, Koh SB, Choi HR, Lee KH, Park CG, Cho SH, Hirvonen A, Strickland PT, Vermeulen R, Hayes RB, Kang DH. Multiple biomarker study in painters in a shipyard in Korea. Mutation Research 540:89-98, 2003.

Mammen JS, Pittman GS, Li Y, Abou-Zahr F, Bejjani BA, Bell DA, Strickland PT, Sutter TR. Single amino acid mutations, but not common polymorphisms, decrease the activity of CYP1B1 against (-)benzo[a]pyrene-7R-trans- 7,8-dihydrodiol. Carcinogenesis 24:1247-1255, 2003.

Lai CH, Liou SH, Shih TS, Tsai PJ, Chen HS, Buckley T, Strickland P, Jaakkola JJK. Urinary 1-hydroxypyrene-glucuronide as a biomarker of exposure to traffic exhausts among highway toll station workers. Arch Environ Health 59:61-69, 2004.

Jackson PE, Kuang SY, Wang JB, Strickland PT, Munoz A, Kensler TW, Qian GS, Groopman JD. Prospective detection of codon 249 mutations in plasma of hepatocellular carcinoma patients. Carcinogenesis 24:1657-1663, 2003.

Kim JH, Sherman ME, Curriero FC, Guengerich FP, Strickland PT, Sutter TR. Expression of cytochromes P450 1A1 and 1B1 in human lung from smokers, non-smokers, and ex-smokers. Toxicol Appld Pharmacol 199:210-219, 2004.

Alberg AJ, Daudt A, Huang HY, Hoffman SC, Comstock GW, Helzlsouer KJ, Strickland PT, Bell DA. N-Acetyl transferase 2 (NAT2) genotypes, cigarette smoking and the risk of breast cancer. Cancer Detect Preven 28:187-193, 2004.

Kamanger F, Strickland PT, Pourshams A, Malekzadeh R, Boffetta P, Roth MJ, Abnet CC, Saadatian-Elahi M, Rakhshani N, Brennan P, Etemadi A, Dawsey SM. High exposure to

polycyclic aromatic hydrocarbons may contribute to high risk of esophageal cancer in northeastern Iran. Anticancer Research 25:425-428, 2005.

Mammen JS, Kleiner HE, DiGiovanni J, Sutter TS, Strickland PT. Coumarins are competitive inhibitors of cytochrome P450 1B1, with equal potency for allelic variants. Pharmacogenetics Genomics 15:183-188, 2005.

Pourshams A, Saadatian-Elahi M, Nouraie M, Malekshah AF, Rakhshani N, Salahi R, Yoonessi A, Semnani S, Islami F, Sotoudeh M, Fahimi S, Sadjadi AR, Nasrollahzadeh D, Aghcheli K, Kamanger F, Abnet CC, Saidi F, Sewram V, Strickland PT, Dawsey SM, Brennan P, Boffetta P, Malekzadeh R. Golestan cohort study of oesophageal cancer: feasibility and first results. Brit J Cancer 92:176-181, 2005.

Lai CH, Liou SH, Shih TS, Tsai PJ, Chen JS, Yang T, Jaakkola JJK, Strickland PT. Exposure to traffic exhausts and oxidative DNA damage. Occupat Environ Med 62:216-222, 2005.

Sastry C, Whitmore E, Breysse P, Morrison WL, Strickland PT. The effect of clinical UVA/B exposures on urinary urocanic acid isomer levels in individuals with Caucasian skin types (II/III). Dermatol Online J 11:1, 2005.

Gaffney SH, Curriero FC, Strickland PT, Glass GE, Helzlsouer KJ, Breysse PN. Influence of geographical location in modeling blood pesticide levels in a community surrounding an EPA Superfund site. Environ Health Perspec 113:1712-1716, 2005.

Sinha R, Kulldorff M, Gunter MJ, Strickland P, Rothman N. Dietary benzo[a]pyrene and risk of colorectal adenoma. Cancer Epidemiol Biomarkers Prev 14:2030-2034, 2005.

Kang DH, Lee KH, Lee KM, Kwon HJ, Hong YC, Cho SH, Strickland PT. Design issues in cross-sectional biomarkers studies:Urinary biomarkers of PAH exposure and oxidative stress. Mutation Research 592:138-146, 2005.

Genkinger JM, Platz EA, Hoffman S, Strickland P, Comstock GW, Helzlsouer KJ. C47T polymorphism in manganese superoxide dismutase (MnSOD), antioxidant intake and all-cause, cancer and cardiovascular disease mortality. Mech Aging Develop 127:371-377, 2006.

Gunier R, Reynolds P, Hurley S, Yerabati S, Hertz A, Strickland P, Horn-Ross P, Anton-Culver H, Bernstein L, Deapen D, Peel D, Pinder R, Ross RK, West D, Wright WE, Ziogas A. Estimating exposure to polycyclic aromatic hydrocarbons: a comparison of survey, biological monitoring and GIS-based methods. Cancer Epidemiol Biomarkers Preven 15:1376-1381, 2006

Fagundes RB, Abnet CC, Strickland PT, Kamanger F, Roth MJ, Taylor PR, Dawsey SM. Higher urine 1-hydroxypyrene glucuronide (1-OHPG) is associated with smoking tobacco, preparing barbeque, and drinking mate in healthy subjects from Rio Grande do Sul, Brazil. BMC Cancer 6:139, 2006.

Abnet CC, Fagundes RB, Strickland PT, Kamangar F, Roth MJ, Taylor PR, Dawsey SM. The influence of genetic polymorphisms in Ahr, CYP1A1, CYP1A2, CYP1B1, GST M1, GST T1, and UGT1A1 on urine 1-hyrdoxypyrene glucuronide concentrations in healthy subjects from Rio Grande do Sul, Brazil. Carcinogenesis 28:112-117, 2007.

Visvanathan K, Crum RM, , Strickland PT, You X, Ruczinski I, Berndt SL, Alberg AJ, Hoffman SC, Comstock GW, Bell DA, Helzlsouer KJ. Alcohol dehydrogenase genetic polymorphisms, low-to-moderate alcohol consumption, and risk of breast cancer. Alcohol Clin Exp Res 31:467-476, 2007.

Engel LS, Laden F, Anderson A, Strickland PT, Blair A, Needham LL, Barr DB, Wolff MS, Helzlsouer K, Hunter DJ, Lan Q, Cantor KP, Comstock GW, Brock JW, Bush D, Hoover RN, Rothman N. Polychlorinated biphenyl levels in peripheral blood and non-Hodgkin’s lymphoma: a report from three cohorts. Cancer Research 67:5545-5552, 2007.

Gunter MJ, Divi RL, Kulldorff M, Vermeulen R, Haverkos KJ, Kuo MM, Strickland PT, Poirier MC, Rothman N, Sinha R. Leukocyte polycyclic aromatic hydrocarbon-DNA adduct formation and colorectal adenoma. Carcinogenesis 28:1426-1429, 2007.

Huang HY, Thuita L, Strickland PT, Hoffman SC, Comstock GW, Helzlsouer KJ. Frequencies of single nucleotide polymorphisms in genes regulating inflammatory responses in a community-based population. BMC Genet 8:7, 2007.